High Level Assembly
- An assembly language developed by Randall Hyde which can use high-level language constructs to aid x86 assembly programmer beginners and advanced assembly developers alike. (Wikipedia article)
- http://en.wikipedia.org/wiki/High_Level_Assembly

MicroAsm
- Micro Assembler IDE includes advanced source editor, 8086 assembler and IDE. All in one combination for beginners. [Freeware]
- http://www.emu8086.com/dr/asm/

Th XAssembler
- An asembler/linker/compiler for Intel x86 family processors. [Free and open source]
- http://xasm.webpark.pl/xasm/

Fresh
- Visual assembly language RAD IDE with built-in FASM assembler. Designed for easy development of big assembly written applications. Self-contained, Fresh is written in Fresh and is fully compatible with FlatAssembler (FASM).
- http://fresh.flatassembler.net/

NewBasic Assembler: NBASM
- Easy-to-use assembler for DOS and the Intel x86 and compatible processors, including support for real mode and protected mode.
- http://www.frontiernet.net/~fys/newbasic.htm

JLOC
- A linker/locator for use when need more control over placement of sections within the image, than a standard linker provides. [Zero-price shareware]
- http://my.execpc.com/~geezer/johnfine/jloc.htm

ALink
- Linker for DOS, Windows. Open source, Artistic License.
- http://alink.sourceforge.net/

YASM
- A complete rewrite of the NASM assembler under BSD License. is designed from the ground up to allow for multiple assembler syntaxes to be supported (eg, NASM, TASM, GAS, etc.) in addition to multiple output object formats and even multiple instruction set
- http://www.tortall.net/projects/yasm/

Niko's Shareware Assembly Tools (dated)
- Collection files and links to toolset for x86 assembler programming.
- http://home.snafu.de/nkomin/html/filese.htm

Flat Assembler
- A self-assembling open-source 80x86 assembler for DOS, Windows and Linux. It supports all 8086-80486/Pentium instructions with MMX, SSE, SSE2 and 3DNow! extensions, can produce output in binary, MZ, PE, COFF or ELF format.
- http://flatassembler.net/

Free Assemblers and Linkers for Programmers
- Lists assemblers, cross-assemblers, linkers, and librarians, where available, for a wide variety of operating systems and processors.
- http://www.thefreecountry.com/compilers/assemblers.shtml

NASM (Netwide Assembler)
- An 80x86 assembler designed for portability and modularity. [Open source, LGPL]
- http://nasm.sourceforge.net/

A86 Assembler, D86 Debugger
- A86: high quality assembler, for x86 processors. D86: screen-oriented program to troubleshoot buggy programs written for DOS; freezes program states, shows values of registers, flags, memory; single steps forward or back. Eric Isaacson Software.
- http://eji.com/a86/